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Main description:
Antibodies tagged with fuorescent markers have been used in histochemistry for over 50 years. Although early applications were focused on the detection of microbial antigens in tissues, the use of immunocytochemical methods now has spread to include the det- tion of a wide array of antigens including proteins, carbohydrates, and lipids from virtually any organism. Today, immunohistochemistry is widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions. The method gains its strength from the extremely sensitive interaction of a specifc antibody with its antigen. For some scientifc areas, books have been published on applications of immu- cytochemical techniques specifc to that area. What distinguished Immunocytochemical Methods and Protocols from earlier books when it was frst published was its broad appeal to investigators across all disciplines, including those in both research and clinical settings. The methods and protocols p- sented in the frst edition were designed to be general in their application; the accompa- ing "Notes" provided the reader with invaluable assistance in adapting or troubleshooting the protocols. These strengths continued to hold true for the second edition and again for the third edition. Since the publication of the frst edition, the application of immuno- tochemical techniques in the clinical laboratory has continued to rise and this third edition provides methods that are applicable to basic research as well as to the clinical laboratory.
Contents:
Immunocytochemical Methods and Protocols, Third Edition
Constance Oliver and Maria Celia Jamur, Eds.
Table of Contents
Preface
Contributors
Part I. Antibody Preparation
Overview of Antibodies for Immunochemistry
Constance Oliver and Maria Celia Jamur
Introduction to the Purification of Antibodies
Ana Cristina Grodzki and Elsa Berenstein
Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration
Ana Cristina Grodzki and Elsa Berenstein
Antibody Purification: Ion - Exchange chromatography
Ana Cristina Grodzki and Elsa Berenstein
Antibody Purification: Affinity Chromatography - Protein A and Protein G Sepharose
Ana Cristina Grodzki and Elsa Berenstein
Conjugation of Fluorochromes to Antibodies
Su-Yau Mao
Biotinylation of Antibodies
Su-Yau Mao
Part II. Tissue Preparation for Light Microscopic Analysis
Cell Fixatives for Immunostaining
Maria Celia Jamur and Constance Oliver
Permeablization of Cell Membranes
Maria Celia Jamur and Constance Oliver
Preparation of Frozen Sections for Analysis
Gary L. Bratthauer
Processing of Cytological Specimens
Gary L. Bratthauer
Processing of Tissue Culture Cells
Gary L. Bratthauer
Processing Of Tissue Specimens
Gary L. Bratthauer
Heat Induced Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections
Laszlo Krenacs, Tibor Krenacs, Eva Stekovics,and Mark Raffeld
Part III: Bright Field Detection Systems
Fluorochromes - Properties and Characteristics
J. Michael Mullins
Direct Immunofluorescent Labeling of Cells
Maria Veronica Davila Pastor
Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells
Mark C. Willingham
Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells
Mark C. Willingham
Fluorescent Visualization of Macromolecules in Drosophila Whole Mounts
Ricardo Guelerman Pinheiro Ramos, Luciana Claudia Herculano Machado, and Livia Maria Rosatto Moda
Overview of Conventional Fluorescence Photomicrography
J. Michael Mullins
Overview of Confocal Microscopy
William D. Swaim
Overview of Laser Microbeam Applications as Related to Antibody Targeting
P. Scott Pine
Immuno-Laser Capture Microdissection of Rat Brain Neurons For Real Time Quantitative PCR
L. Scot Bastian and Denis G. Baskin
Overview of Antigen Detection through Enzymatic Activity
Gary L. Bratthauer
The Peroxidase-antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods
Gary L. Bratthauer
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
Gary L. Bratthauer
Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue
Mark Raffeld and Elaine S. Jaffe
Multiple Antigen Immunostaining Procedures
Tibor Krenacs, Laszlo Krenacs and Mark Raffeld
Immunoenzymatic Quantitative Analysis of Antigens
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: April, 2012
Pages: 428
Weight: 810g
Availability: Available
Subcategories: Immunology, Pathology
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