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Main description:
In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential. In Macrophages and Dendritic Cells: Methods and Protocols, expert researchers contribute laboratory protocols involving these two vital cell types functioning at the junction of the innate and acquired immune systems. The volume delves first into isolation and cell culturing then continues with topics such as phagocytosis, genetic manipulation, macrophage activation, and lipid signaling. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Macrophages and Dendritic Cells: Methods and Protocols provides a timely and useful guide for both seasoned investigators and neophytes pursuing this imperative field of study.
Contents:
1. Generation of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors
Georgia Schafer and Gordon D. Brown
2. Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite Interactions
Alessandra Mortellaro, Matteo Urbano, Stefania Citterio, Maria Foti, Francesca Granucci, and Paola Ricciardi-Castagnoli
3. Macrophage Activation: Classical Versus Alternative
Andrea Classen, Jorge Lloberas, and Antonio Celada
4. Analysis of Macrophage Phagocytosis: Quantitative Assays of Phagosome Formation and Maturation Using High-Throughput Fluorescence Microscopy
Benjamin E. Steinberg and Sergio Grinstein
5. Detection of Activated Rab7 GTPase with an Immobilized RILP Probe
Jim Sun, Ala-Eddine Deghmane, Cecilia Bucci, and Zakaria Hmama
6. Probing In Vivo Origins of Mononuclear Phagocytes by Conditional Ablation and Reconstitution
Chen Varol, Limor Landsman, and Steffen Jung
7. Multivalent Protein Probes for the Identification and Characterization of Cognate Cellular Ligands for Myeloid Cell Surface Receptors
Hsi-Hsien Lin, Gin-Wen Chang, Yi-Shu Huang, Cheng-Chih Hsiao, Martin Stacey, and Siamon Gordon
8. Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose Receptor
Luisa Martinez-Pomares
9. The Expression of Exogenous Genes in Macrophages: Obstacles and Opportunities
Xia Zhang, Justin P. Edwards, and David M. Mosser
10. Genetic Modification of Murine Dendritic Cells by RNA Transfection
Maurizio Ceppi, Enrico Schmidt, and Philippe Pierre
11. Recording Phagosome Maturation Through the Real-Time, Spectrofluorometric Measurement of Hydrolytic Activities
Robin M. Yates, Albin Hermetter, and David G.Russell
12. Derivation and Characterization of Murine Alternatively Activated (M2) Macrophages
Victor W.H. Ho and Laura M. Sly
13. Isolation and Culture of Human Hematopoietic Progenitors for Studies of Dendritic Cell Biology
Mattias Svensson
14. Generation, Culture and Flow-Cytometric Characterization of Primary Mouse Macrophages
Ulrike Schleicher and Christian Bogdan
15. Analyzing Classical and Alternative Macrophage Activation in Macrophage/Neutrophil-Specific IL-4 Receptor-Alpha-Deficient Mice
Frank Brombacher, Berenice Arendse, Reagon Peterson, Alexandra Hoelscher, and Christoph Hoelscher
16. Analysis of Matrix Metalloproteinase Secretion by Macrophages
Paul T. Elkington, Justin A. Green, and Jon S. Friedland
17. Identification of Pathogen Receptors on Dendritic Cells to Understand their Function and to Identify New Drug Targets
Lot de Witte, Marein A.W.P. de Jong, Jeroen den Dunnen, Yvette van Kooyk, and Teunis B.H. Geijtenbeek
18. Stable Lentiviral Vector-Mediated Gene Silencing in Human Monocytic Cell Lines
Jimmy S. Lee and Neil E. Reiner
19. Use of Fluorescent Probes to Detect Lipid Signaling Intermediates in Macrophages
Vivian Kwok, Eric Vachon, and Gregory P. Downey
20. Large Scale Phagosome Preparation
Adrien F. Vinet and Albert Descoteaux
21. Coordinate Regulation of Sphingosine Kinase and Actin Dynamics
Shankar S. Iyer and David J. Kusner
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2014
Pages: 368
Weight: 808g
Availability: Available
Subcategories: Immunology
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