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Estrogen Receptors

Methods and Protocols

Series: Methods in Molecular Biology

by Kathleen M. Eyster

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Main description:

This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen.

The chapter 'Bioinformatics Analysis of Estrogen-Responsive Genes' is open access under a CC BY 4.0 license.

Contents:

1. The Estrogen Receptors: An Overview from Different Perspectives
Kathleen M. Eyster

2. Competitive Binding Assay for the G-Protein Coupled Receptor 30 (GPR30) or G-Protein Coupled Estrogen Receptor (GPER)
Thomas Thekkumkara, Russell Snyder, and Vardan T. Karamyan

3. The Use of Real Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression
Michelle L. Booze and Kathleen M. Eyster

4. Bioinformatics Analysis of Estrogen-Responsive Genes
Adam E. Handel

5. Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen
Kwi Hye Koh and Hyunyoung Jeong

6. Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors
Meike Wagner, Johannes Jung, Michael Koslowski, OEzlem Tureci, Vijay K. Tiwari, and Ugur Sahin
7. Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells
Jing Lin and Zhenmin Lei

8. Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast Cancer
Kelly A. Holmes, Gordon D. Brown, and Jason S. Carroll

9. RNA-Seq Experiment and Data Analysis
Hanquan Liang and Erliang Zeng

10. DNA Microarray Analysis of Estrogen-Responsive GenesKathleen M. Eyster

11. Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two Dimensional Liquid Chromatography and Tandem Mass Spectrometry
Eduardo A. Callegari

12. Assessment of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate Endometrium, Placenta, and Fetal Adrenal in Response to Estrogen
Thomas W. Bonagura, Jeffery S. Babischkin, Gerald J. Pepe and Eugene D. Albrecht
13. Co-Localization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by Confocal Microscopy
Araceli Morales, Raquel Marin, Jorge Marrero-Alonso, Alicia Boto, and Mario Diaz

14. Live Cell Imaging of the Estrogen Receptor by Total Internal Reflection Fluorescence Microscopy
Kassandra Kisler and Reymundo Dominguez

15. In Situ Hybridization of Estrogen Receptors and and GPER in the Human Testis
D. Fietz, M. Bergmann, and K. Hartmann

16. Purification of Histone Lysine Methyltransferase SMYD2 and Co-Crystallization with a Target Peptide from Estrogen Receptor a
Yuanyuan Jiang, Joshua Holcomb, Nicholas Spellmon, and Zhe Yang

17. Gold Nanoparticle-Based Foerster Resonance Energy Transfer (FRET) Analysis of Estrogen Receptor: DNA Interaction
Xiaodi Su, Khin Moh Moh Aung, Steven Lukman, and Bin Liu

18. Analysis of Interaction of Estradiol with Estrogen Receptor by NMR Spectroscopy
M.K. Thakur and V. Paramanik

19. Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction between the Estrogen Receptor Alpha and Co-Activators: The Case of Calmodulin
Emeric Miclet, Sandrine Bourgoin-Voillard, Cillian Byrne, and Yves Jacquot

20. Surface Plasmon Resonance Study of Cooperative Interactions of Estrogen Receptor and Specificity Protein 1 with Composite DNA Elements
Xiaodi Su and Hong Yan Song

21. Tips and Tricks for Successful Application of Statistical Methods to Biological Data
Evelyn Schlenker

22. The Synonymous Ala87 Mutation of Estrogen Receptor Alpha Modifies Transcriptional Activation through Both ERE and AP1 Sites
Tamara Fernandez-Calero, Gilles Flouriot, and Monica Marin

<23. Selective Estrogen Receptor Modulators and the Tissue-Selective Estrogen Complex: Analysis of Cell Type-Specific Effects using In Vivo Imaging of a Reporter Mouse Model
Sara Della Torre and Paolo Ciana

24. Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model
Daniel A. Gorelick, Caroline Lucia Pinto, Ruixin Hao, and Maria Bondesson

25. Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo CultureNatalija Eigeliene, Risto Erkkola, and Pirkko Harkoenen

26. Estrogen Receptor Agonists and Antagonists in the Yeast Estrogen Bioassay
Si Wang and Toine F. H. Bovee

27. Silencing Estrogen Receptor- with siRNA in the Intact Rodent Brain
Ana C. Ribeiro, Anders Agmo, Sergei Musatov, and Donald W. Pfaff

28. Silencing Estrogen Receptor- with siRNA in Cultured Cells
Ren-Jun Hsu and Jui-Ming Liu

29. Experimental Procedures for Demonstration of MicroRNA Mediated Enhancement of Functional Neuroprotective Effects of Estrogen Receptor Agonists
Mrinmay Chakrabarti and Swapan K. Ray

30. Expression Profiles of Estrogen-Regulated MicroRNAs in Breast Cancer Cells
Anne Katchy and Cecilia Williams

31. Estradiol-Induced Transcriptional Regulation of Long Non-Coding RNA, HOTAIR
Arunoday Bhan and Subhrangsu S. Mandal

32. Detection and Functional Analysis of Estrogen Receptor Phosphorylated at Serine 216 in Mouse Neutrophils
Sawako Shindo, Rick Moore, and Masahiko Negishi

33. Estrogen Receptor- Knockout Mice
Per Antonson, Patricia Humire, and Jan-Ake Gustafsson

34. Detection of Endogenous Selective Estrogen Receptor Modulators such as 27-Hydroxycholesterol
Erik R. Nelson

35. Phytoestrogens Activate the Estrogen Receptor in HepG2 CellsLynne A. Kelly

36. Detection of the Phosphorylation of the Estrogen Receptor a as an Outcome of GPR30 Activation
Sara Clark, Kevin Pollard , Jennifer Rainville, and Nandini Vasudevan

37. GPER Mediates Non-Genomic Effects of Estrogen
Marco Pupo, Marcello Maggiolini, and Anna Maria Musti

38. GPER/GPR30 Knockout Mice: Effects of GPER on Metabolism
Geetanjali Sharma and Eric R. Prossnitz

39. Analysis of G-Protein Coupled Receptor 30 (GPR30) on Endothelial Inflammation Subhadeep Chakrabarti and Sandra T. Davidge

40. Atherosclerosis and Vascular Biologic Responses to Estrogens: Histologic, Immunohistochemical, Biochemical, and Molecular Methods
Thomas C. Register, Susan E. Appt, and Thomas B. Clarkson

41. Assessing Direct Vascular Actions of Estrogens
Douglas S. Martin, and Kathleen M. Eyster

42. Molecular Design, Synthesis, and Evaluation of SNIPER(ER) that Induces Proteasomal Degradation of ER
Keiichiro Okuhira, Yosuke Demizu, Takayuki Hattori, Nobumichi Ohoka, Norihito {Itoh, 2010 #4}Shibata, Masaaki Kurihara, and Mikihiko Naito

43. Regulation of Activation Induced Deaminase (AID) by Estrogen
Siim Pauklin

PRODUCT DETAILS

ISBN-13: 9781493931262
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2015
Pages: 576
Weight: 652g
Availability: Available
Subcategories: Biochemistry, Endocrinology

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