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Chemotaxis

Methods and Protocols

Series: Methods in Molecular Biology

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by Tian Jin and Dale Hereld

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Main description:

Fundamental to the development and vital functions of organisms, the migration of motile cells due to the detection of shallow gradients of specific chemical signals in their environments, or chemotaxis, can be clearly seen as a major force in cell biology. In Chemotaxis: Methods and Protocols, expert researchers in the field provide state-of-the-art methods for investigating cell migration behaviors, studying molecular components involved in detecting extracellular signals and directing cell movement, visualizing spatiotemporal dynamics of the components in signaling networks of chemotaxis in real time, and constructing quantitative models that simulate chemoattractant-induced cell responses. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Chemotaxis: Methods and Protocols serves scientists with practical guidance on the diverse methodologies that are currently propelling chemotaxis research forward in order to further our understanding of this vital biological system.

Feature:

Describes a breadth of uniquely suited model systems for analysis of chemotaxis, including bacteria, Dictyostelium, yeast, Drosophila, zebrafish, neurons, and immune and tumor cells

Examines phenomena related to chemotaxis, including chemotropism, phototaxis, and electrotaxis

Details the application of cutting-edge technologies for stimulating cells with chemoattractants and analyzing their resulting cellular behaviors

Covers a variety of state-of-the-art microscopic methods for visualizing the migration of cells in tissues and the spatiotemporal dynamics of chemotaxis signaling molecules

Provides step-by-step functional assays for a variety of components involved in chemotaxis, including cell-surface receptors, downstream effectors, and cytoskeletal components

Explores the quantitative description of chemoattractant gradients and computational approaches for modeling the signaling networks that underlie gradient sensing

Back cover:

Contents:

1. Microfluidic Techniques for the Analysis of Bacterial Chemotaxis
Derek L. Englert, Arul Jayaraman, and Michael D. Manson

2. Prokaryotic Phototaxis
Wouter D. Hoff, Michael A. van der Horst, Clara B. Nudel, and Klaas J. Hellingwerf

3. Photoorientation in Photosynthetic Flagellates
Donat-Peter Häder and Michael Lebert

4. Dictyostelium Slug Phototaxis
Sarah J. Annesley and Paul R. Fisher

5. Electrotaxis and Wound Healing: Experimental Methods to Study Electric Fields as a Directional Signal for Cell Migration
Guangping Tai, Brian Reid, Lin Cao, and Min Zhao

6. Chemotropism During Yeast Mating
Peter J. Follette and Robert A. Arkowitz

7. Group Migration and Signal Relay In Dictyostelium
Paul W. Kriebel and Carole A. Parent

8. Quantitative Analysis of Distal Tip Cell Migration in C. elegans
Myeongwoo Lee and Erin J. Cram

9. Inflammation and Wound Healing in Drosophila
Brian Stramer and Will Wood

10. Neutrophil Motility In Vivo Using Zebrafish
Jonathan R. Mathias, Kevin B. Walters, and Anna Huttenlocher

11. Chemotaxis in Neutrophil-like HL-60 Cells
Arthur Millius and Orion D. Weiner

12. Chemokine Receptor Dimerization and Chemotaxis
José Miguel Rodríguez-Frade, Laura Martinez Muñoz, Borja L. Holgado, and Mario Mellado

13. Intravital Two-Photon Imaging of Adoptively Transferred B Lymphocytes in Inguinal Lymph Nodes
Chung Park, Il-Young Hwang, and John H. Kehrl

14. Breast Cancer Cell Movement: Imaging Invadopodia by TIRF and IRM Microscopy
Xuehua Xu, Peter Johnson, and Susette C. Mueller

15. In vivo Assay for Tumor Cell Invasion
Lorena Hernandez, Tatiana Smirnova, Jeffrey Wyckoff, John Condeelis, and Jeffrey E. Segall

16. Quantitative Studiesof Neuronal Chemotaxis in 3D
William J. Rosoff, Ryan G. McAllister, Geoffrey J. Goodhill, and Jeffrey S. Urbach

17. Assays for Chemotaxis and Chemoattractant-Stimulated TorC2 Activation and PKB Substrate Phosphorylation in Dictyostelium
Yoichiro Kamimura, Ming Tang, and Peter Devreotes

18. Biochemical Responses to Chemoattractants in Dictyostelium: Ligand-Receptor Interactions and Downstream Kinase Activation
Xin-Hua Liao and Alan R. Kimmel

19. Quantifying In Vivo Phosphoinositide Turnover in Chemotactically-Competent Dictyostelium Cells
Nadine Pawolleck and Robin S.B. Williams

20. In Vivo Measurements of Cytosolic Calcium in Dictyostelium discoideum
Claire Y. Allan and Paul R. Fisher

21. Chemokine Receptor Signaling and HIV Infection
Yuntao Wu

22. Spatiotemporal Stimulation of Single Cells Using Flow Photolysis
Carsten Beta

23. Spatio-Temporal Regulation of Ras-GTPases During Chemotaxis
Atsuo T. Sasaki and Richard A. Firtel

24. FRAP Analysis of Chemosensory Components of Dictyostelium
Carrie A. Elzie and Chris Janetopoulos

25. Monitoring Dynamic GPCR Signaling Events Using Fluorescence Microscopy, FRET Imaging and Single Molecule Imaging
Xuehua Xu, Joseph A. Brzostowski, and Tian Jin

26. Imaging Actin Cytoskeleton Dynamics in Dictyostelium Chemotaxis
Günther Gerisch

27. Analysis of Actin Assembly by In Vitro TIRF Microscopy
Dennis Breitsprecher, Antje K. Kiesewetter, Joern Linkner, and Jan Faix

28. Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Living Dictyostelium Cells
Yukihiro Miyanaga, Satomi Matsuoka, and Masahiro Ueda

29. Imaging B-cell Receptor Signaling by Single Molecule Techniques
Pavel Tolar and Tobias

PRODUCT DETAILS

ISBN-13: 9781607611974
Publisher: Springer (Humana Press)
Publication date: September, 2009
Pages: 538
Weight: 1499g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry

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CUSTOMER REVIEWS

Average Rating

From the reviews: “The purpose is to provide an overview of and practical guidance on the diverse methodologies involved in chemotaxis research. … Each chapter includes an introduction to the topic, lists of necessary materials and reagents, laboratory protocol, and notes on troubleshooting and avoiding pitfalls, followed by current references. … This will serve as a valuable resource for students and scientists seeking practical guidance on diverse methodologies in chemotaxis research.” (Omer Iqbal, Doody’s Review Service, April, 2010)