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Main description:
A collection of cutting-edge techniques for analyzing genotoxic exposure and detecting the resulting biological effects-including endogenous metabolites-up to and including the development of cancer. The authors emphasize analytical methods that can be specifically applied to human populations and patients. Among the applications detailed are the analysis of interactions between such cellular macromolecules as DNA and proteins and chemical and physical agents, the assessment of medically relevant toxicity, and the characterization of genetic alterations induced in transgenic animals by in vivo systems. There are also methods for the analysis of genotoxic exposure during gene expression, of cytotoxicity caused by the induction of apoptosis, of genetic alterations in reporter genes and oncogenes, early (premalignant) detection of altered oncogenes, and of individual variation in biotransformation and DNA repair capacity.
Contents:
Part I. Analysis of DNA Adducts
32P-Postlabeling Analysis of DNA Adducts
David H. Phillips, Alan Hewer, and Volker M. Arlt
Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot
Masako Ochiai, Takashi Sugimura, and Minako Nagao
DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry
Karen H. Dingley, Esther A. Ubick, John S. Vogel, and Kurt W. Haack
Fluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated Tadpoles
Iovanna Pandelova, Stephen R. Hewitt, and John B. Hays
Analysis of DNA Strand Cleavage at Abasic Sites
Walter A. Deutsch and Vijay Hegde
Part II. Detection of Chromosomal and Genome-Wide Damage
Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization Probes
Pataje G. S. Prasanna and William F. Blakely
Mutagen-Induced Chromatid Breakage as a Marker of Cancer Risk
Xifeng Wu, Yun-Ling Zheng, and T. C. Hsu
Flow Cytometric Analysis of Micronuclei in Erythrocytes, Jan Grawe
The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage
Gunter Speit and Andreas Hartmann
Computerized Image Analysis Software for the Comet Assay
R. C. Chaubey
The Comet-FISH Technique: A Tool for Detection of Specific DNA Damage and Repair
Alexander Rapp, Michael Hausmann, and Karl Otto Greulich
DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresis
Nina Joshi and Stephen G. Grant
Part III. Detection and Characterization of Surrogate Gene Mutation
Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes
Vasily N. Dobrovolsky, Joseph G. Shaddock, and Robert H.Heflich
Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems
Roy R. Swiger
Methods for Detecting Somatic Mutations In Vitro: The Human T-Cell Cloning Assay Selecting for HPRT Mutants
Sai-Mei Hou
Molecular Analysis of Mutations in the Human HPRT Gene
Phouthone Keohavong, Liqiang Xi, and Stephen G. Grant
Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes
James C. Fuscoe
The GPA In Vivo Somatic Mutation Assay
Stephen G. Grant
Flow Cytometric Measurement of Mutant T Cells With Altered Expression of TCR: Detecting Somatic Mutations in Humans and Mice
Seishi Kyoizumi, Yoichiro Kusunoki, and Tomonori Hayashi
Part IV. Detection and Characterization of Cancer Gene Mutation
Mutation Screening of the TP53 Gene by Temporal Temperature Gradient Gel Electrophoresis
Therese Sorlie, Hilde Johnsen, Phuong Vu, Guro Elisabeth Lind, Ragnhild Lothe, and Anne-Lise Borresen-Dale
Analysis of K-RAS and P53 Mutations in Sputum Samples
Weimin Gao and Phouthone Keohavong
Allele-Specific Competitive Blocker-PCR Detection of Rare Base Substitution
Barbara L. Parsons, Page B. McKinzie, and Robert H. Heflich
Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection: Limitations and Solutions
Vibhuti Gupta, Reetakshi Arora, Anand Ranjan, Narendra K. Bairwa, Dheeraj K. Malhotra, P. T. Udhayasuriyan, Anjana Saha, and Ramesh Bamezai
Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions
Toshinari Minamoto
Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR
Michael J. Villalobos, Christopher J. Betti, and Andrew T. M. Vaughan
Part V. Analysis of DNA Repair Mechanisms
Microsatellite
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2011
Pages: 504
Weight: 765g
Availability: Available
Subcategories: Biochemistry, Pharmacology
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