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Main description:
Neuroproteomics: Methods and Protocols presents experimental details for applying proteomics to the study of the central nervous system (CNS) and its dysfunction through trauma and disease. The target audience includes clinical or basic scientists who look to apply proteomics to the neurosciences. Often researchers hear of proteomics without an adequate explanation of the methodology and inherent limitations. This volume conveys where proteomic methodology is in its application to CNS research and what results can be expected. We also address clinical translation of neuroproteomics, specifically in the area of biomarker research. The inception of neuroproteomics capitalized on rapid progress in large-molecule mass spectrometry over the last decade. Two seminal advances have spurred research - development of reliable polypeptide ionization processes and bioinformatics to rapidly process tandem mass spectra for peptide identification and quantification. What has followed is the exponential application of mass spectrometry to proteome characteri- tion across biological and biomedical disciplines. Arguably, the most elaborate proteomic implementation is in studying the CNS, the most enigmatic and complex animal system. Neuroscience is characterized by grandiose questions - what is consciousness, how does thought or memory work. Neuroproteomics researchers, however, have pri- rily involved themselves dysfunction, based on a pressing need (and invariably funding), in answering questions on CNS dysfunction, based on a pressing need (and invariably funding), and because such questions hold more accessible answers. Dysfunction is readily contrasted against normal function and presumably produces a lasting differential protein signature.
Contents:
1. The Methodology of Neuroproteomics
Andrew K. Ottens
Part I: Disease Models in Neuroproteomics
2. Modeling Cerebral Ischemia in Neuroproteomics
Jitendra R. Dave, Anthony J. Williams, Changping Yao, X-C. May Lu, and Frank C. Tortella
3. Clinical and Model Research of Neurotrauma
Andras Buki, Erzsebet Kovesdi, Jozsef Pal, and Endre Czeiter
4. Neuroproteomic Methods in Spinal Cord Injury
Anshu Chen and Joe E. Springer
5. Modeling Substance Abuse for Applications in Proteomics
Scott E. Hemby and Nilesh Tannu
6. Protein Aggregate Characterization in Models of Neurodegenerative Disease
Andrew T. N. Tebbenkamp and David R. Borchelt
Part II: Sub-Proteome Separations and Neuroproteomic Analysis
7. Sub-Proteome Processing: Isolation of Neuromelanin Granules from the Human Brain
Florian Tribl
8. Proteomic Analysis of Protein Phosphorylation and Ubiquitination in Alzheimer's Disease
Stefani N. Thomas, Diane Cripps, and Austin J. Yang
9. Proteomics Identification of Carbonylated and HNE-Bound Brain Proteins in Alzheimer's Disease
Rukhsana Sultana and D. Allan Butterfield
10. Mass Spectrometric Identification of in vivo Nitrotyrosine Sites in the Human Pituitary Tumor Proteome
Xianquan Zhan and Dominic M. Desiderio
11. Improved Enrichment and Proteomic Analysis of Brain Proteins with Signaling Function by Heparin Chromatography
Kurt Krapfenbauer and Michael Fountoulakis
12. Calmodulin Binding Proteome in the Brain
Zhiqun Zhang, Firas H. Kobeissy, Andrew K. Ottens, Juan A. Martinez, and Kevin K.W. Wang
Part III: Neuroproteomic Methodology and Bioinformatics
13. Separation of the Neuroproteome by IonExchange Chromatography
Brian F. Fuller and Andrew K. Ottens
14. iTRAQ-Based Shotgun Neuroproteomics
Tong Liu, Jun Hu, and Hong Li
15. Methods in Drug Abuse Neuroproteomics: Methamphetamine Psychoproteome
Firas H. Kobaissy, Zhiqun Zhang, Shankar Sadasivan, Mark S. Gold, and Kevin K.W. Wang
16. Shotgun Protein Identification and Quantification by Mass Spectrometry In Neuroproteomics
Bingwen Lu, Tao Xu, Robin Park, Daniel B. McClatchy, Lujian Liao, and John R. Yates III
Part IV: Biofluid Analysis and Clinical Translation
17. Identification of Glycoproteins in Human Cerebrospinal Fluid
Hye Jin Hwang, Thomas Quinn, and Jing Zhang
18. Mass Spectrometric Analysis of Body Fluids for Biomarker Discovery
David M. Good and Joshua J. Coon
19. Traumatic Brain Injury Biomarkers: From Pipeline to Diagnostic Assay Development
Monika W. Oli, Ronald L. Hayes, Gillian Robinson, and Kevin K.W. Wang
20. Translation of Neurological Biomarkers to Clinically Relevant Platforms
Ronald L. Hayes, Gillian Robinson, Uwe Muller, and Kevin K.W. Wang
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2009
Pages: 334
Weight: 921g
Availability: Available
Subcategories: Neurology, Neuroscience, Physiology
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